中文名稱

正常人結(jié)腸上皮細(xì)胞；FHC

組織

結(jié)腸

形態(tài)特性

上皮樣

生長特性

貼壁生長

背景特征

Characteristics: Forms solid tumors after transplantation into SCID mice.Doubling time: ~62 hours (PubMed=6519668).Karyotypic information: Is hypotriploid， has >20 copies of the 8q23~8q24.3 region containing MYC.Omics: Transcriptome analysis.Derived from sampling site: Fetal colon.

培養(yǎng)體系

he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium， add the following components to the base medium:extra 10 mM HEPES (for a final conc. of 25 mM)10 ng/ml cholera toxin0.005 mg/ml insulin0.005 mg/ml transferrin100 ng/ml hydrocortisone20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)Fetal Bovine Serum， 10% final conc (ATCC 30-2020)

培養(yǎng)條件

空氣，95%；CO2，5%。 溫度：37℃

傳代方法

建議第一次1:2傳代   換液頻率2~3次/周

凍存條件

90FBS+10%DMSO,推薦無血清凍存液（CX001）

產(chǎn)品使用

僅限于科學(xué)研究